Plexora
Getting started

Interface overview

The parts of the Plexora window, and the words the app uses for them.

The viewer is one image with a sidebar beside it. The sidebar holds everything about how the image is drawn; the menu bar opens tools and pages.

The Plexora viewer: the menu bar along the top, the sidebar with the Image card, Layers and the Cells row on the left, and a multiplexed tissue image filling the rest of the window
The viewer, with a multiplexed image, its mask and a cell table.

The parts of the window

  1. Menu bar. File (Import Sample…, Samples…, Export Image, Settings…), Tools (the plugins), View (Rotate & Flip, Scalebar) and Help.
  2. Sidebar header. One-click buttons for some tools, and Saved views.
  3. Image card. The image's channels: which are on, their colours and contrast. See Channels and contrast.
  4. Layers. Anything drawn over the image, one card each. See Layers.
  5. Cells row. How cells are drawn: None, Centroids, Outlines or Filled, plus HD. See Segmentation and cell data.
  6. The image. Drag to pan, scroll to zoom. Zoom and Home buttons sit on the left edge; the overview map opens from the bottom-left corner.
  7. Previous / Next sample. Top right, when the sample is in a dataset (B and N).
  8. Depth strip. Along the bottom, only for a z-stack or a time series. See Image viewer.

Tool cards open in the sidebar and stay open side by side. ⌘\ (Ctrl+\ on Windows and Linux) collapses the sidebar.

Words the app uses

WordMeans
SampleOne image and everything attached to it. Python and the command line call it a project.
DatasetA named group of samples, such as a cohort. It holds names, not data.
ChannelOne marker in the image, shown in a colour you choose.
LayerAnything drawn over the image: another image, a mask, points.
Segmentation maskA label image with one number per cell, made before Plexora.
Cell tableOne row per cell. Its columns are markers (intensities) or metadata (everything else).
ToolA plugin, opened from the Tools menu.
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